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外2019 (IF 2.472 NEURAL REGENERATION RESEARCH)高频rTMS联合神经妥乐平对损伤的大鼠坐骨神经再生的影响Jie Chen -Xianju Zhou -Rong-Bin Sun
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1 NEURAL REGENERATION RESEARCH www.nrronline.org 2 mm 2 mm 10 mm Distal Proximal RESEARCH ARTICLE Effect of the combination of high-frequency repetitive magnetic stimulation and neurotropin on injured sciatic nerve regeneration in rats Correspondence to: Xian-Ju Zhou, PhD, xianju_zhou@yahoo.com; Rong-Bin Sun, MS, rongbin_sun@sina.com. orcid: 0000-0003-1744-556X (Xian-Ju Zhou) 0000-0003-2900-7276 (Rong-Bin Sun) doi: 10.4103/ Received: November 10, 2018 Accepted: March 5, 2019 Jie Chen 1, Xian-Ju Zhou 2, 3, *, Rong-Bin Sun 1, * 1 Department of Orthopedics, the Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, Changzhou, Jiangsu Province, Chi- na 2 Laboratory of Neurological Diseases, Department of Neurology, the Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, Changzhou, Jiangsu Province, China 3 Department of Neurology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China Funding: This study was funded by the National Natural Science Foundation of China, No. 81471338 and No. 81671284 (to XJZ); the Changzhou Science & Technology Program of China, No. CE20145045 (to XJZ); the Changzhou High-Level Medical Talents Training Project of China, No. 2016CZLJ018 (to XJZ). Abstract Repetitive magnetic stimulation is effective for treating posttraumatic neuropathies following spinal or axonal injury. Neurotropin is a potential treatment for nerve injuries like demyelinating diseases. This study sought to observe the effects of high-frequency repetitive mag- netic stimulation, neurotropin and their combined use in the treatment of peripheral nerve injury in 32 adult male Sprague-Dawley rats. To create a sciatic nerve injury model, a 10 mm-nerve segment of the left sciatic nerve was cut and rotated through 180° and each end restored continuously with interrupted sutures. The rats were randomly divided into four groups. The control group received only a reversed auto- graft in the left sciatic nerve with no treatment. In the high-frequency repetitive magnetic stimulation group, peripheral high-frequency repetitive magnetic stimulation treatment (20 Hz, 20 min/d) was delivered for 10 consecutive days after auto-grafting. In the neurotropin group, neurotropin therapy (0.96 NU/kg per day) was administrated for 10 consecutive days after surgery. In the combined group, the com- bination of peripheral high-frequency repetitive magnetic stimulation (20 Hz, 20 min/d) and neurotropin (0.96 NU/kg per day) was given for 10 consecutive days after the operation. The Basso-Beattie-Bresnahan locomotor rating scale was used to assess the behavioral recovery of the injured nerve. The sciatic functional index was used to evaluate the recovery of motor functions. Toluidine blue staining was per- formed to determine the number of myelinated fibers in the distal and proximal grafts. Immunohistochemistry staining was used to detect the length of axons marked by neurofilament 200. Our results reveal that the Basso-Beattie-Bresnahan locomotor rating scale scores, sciatic functional index, the number of myelinated fibers in distal and proximal grafts were higher and axon lengths were longer in the high-fre- quency repetitive magnetic stimulation, neurotropin and combined groups compared with the control group. These measures were not significantly different among the high-frequency repetitive magnetic stimulation, neurotropin and combined groups. Therefore, our results suggest that peripheral high-frequency repetitive magnetic stimulation or neurotropin can promote the repair of injured sciatic nerves, but their combined use seems to offer no significant advantage. This study was approved by the Animal Ethics Committee of the Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, China on December 23, 2014 (approval No. 2014keyan002-01). Key Words: nerve regeneration; repetitive magnetic stimulation; neurotropin; peripheral nerve injuries; sciatic nerve; myelinated nerve fibers; axon; trauma; neurological rehabilitation; neural regeneration Chinese Library Classification No. R454; R364 Graphical Abstract Peripheral high-frequency repetitive magnetic stimulation (HF-rMS) or neurotropin promotes peripheral nerve regeneration

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