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外2019 (IF 2.072 Brain and Behavior)一种rTMS刺激小鼠和大鼠的固定装置Chengliang Zhang-Xianju Zhou
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Brain and Behavior. 2019;00:e01305. | 1 of 7 https://doi.org/10.1002/brb3.1305 wileyonlinelibrary.com/journal/brb3 1 | BACKGROUND Repetitive transcranial magnetic stimulation (rTMS) has been widely used for the treatment of neurological and psychiatric diseases, such as Alzheimer's disease, Parkinson's disease, epilepsy, stroke, depression and anxiety disorder, and sleep disorder (Cao et al., 2018; Feng, Zhang, Zhang, Wen, & Zhou, 2019; Lefaucheur et al., 2014; Zhang et al., 2019). However its therapeutic mechanisms are not completely clear. Previous studies have used rodent animals to investigate its potential cellular and molecular mechanisms related to synaptic plasticity (Guo, Lou, Han, Deng, & Huang, 2017; Ji et al., 2013; Tang, Thickbroom, & Rodger, 2015). Unlike humans, it is not practical to keep animals (in‐ cluding mouse or rat) stationary during the delivery of longer rTMS pe‐ riod. Therefore, it is necessary to take some measures to restrain the animals. In previous literature, investigators used the anesthesiology method (Sasso et al., 2016; Sykes et al., 2016), or by hand (Hesselberg, Wegener, & Buchholtz, 2016; Lim, Lee, Yoo, & Kwon, 2014; Sasso et al., 2016), or cloth, bag and straps (Ljubisavljevic et al., 2015); Tang et al., 2018), or some undefined devices (Guo et al., 2014). In animals, rTMS is often used 1–2 times each day (for minutes to tens of minutes each Received: 18 December 2018 | Revised: 7 April 2019 | Accepted: 9 April 2019 DOI: 10.1002/brb3.1305 M E T H O D S Restraint devices for repetitive transcranial magnetic stimulation in mice and rats Chengliang Zhang1 | Rulan Lu1 | Linxiao Wang1 | Wenwei Yun1 | Xianju Zhou1,2 This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. 2019 The Authors. Brain and Behavior published by Wiley Periodicals, Inc. 1Laboratory of Neurological, Department of Neurology, The affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, Changzhou, China 2Department of Neurology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China Correspondence Xianju Zhou, Laboratory of Neurological, Department of Neurology, The affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, Changzhou, 213003, Jiangsu, China. Email: xianju_zhou@yahoo.com Funding information National Natural Science Foundation of China, Grant/Award Number: 81471338 and 81671284; the Changzhou High‐Level Medical Talents Training, Grant/Award Number: NO.2016CZLJ018; Changzhou Sci & Tech Program Grant, Grant/Award Number: CE20145045 Abstract Introduction: Repetitive transcranial magnetic stimulation has been widely used for the treatment of neurological and psychiatric diseases. Rodent animals includ‐ ing mice and rats are often used to investigate the potential cellular and molecular mechanisms for the therapeutic effects of repetitive transcranial magnetic stimula‐ tion. So far there is no report about an easy‐to‐use device to restrain rodent animals for repetitive transcranial magnetic stimulation. Methods and Results: We introduced the design and use of the restraint device for mice or rats. In the mouse device, western blot and real‐time PCR analysis showed that , in stimulated mouse frontal cortex, 10 Hz high frequency stimulation for 10 ses‐ sions resulted in enhanced expression of NR2B‐containing N‐methyl‐D‐aspartic acid receptors and reduced α1 subunit of inhibitory GABAA receptors, whereas 0.5 Hz low frequency stimulation for 10 sessions caused decreased expression of NR2B subunit and increased α1 subunit of GABAA receptors. In the rat device, measures of motor evoke potentials indicated that 10 Hz stimulation for 10 sessions increased the excit‐ ability of stimulated cortex, whereas 0.5 Hz for 10 sessions reduced it. Conclusions: These results suggested the effectiveness of the devices. Thus, the two devices are practical and easy‐to‐use to investigate the mechanisms of repetitive transcranial magnetic stimulation. K E Y W O R D S mouse, rat, repetitive transcranial magnetic stimulation, restraint device

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