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外2017 (IF 6.19)rTMS通过增加前扣带回PTENAkt信号通路中的谷氨酸能传递和突触可塑性来改善PTSD大鼠症状Hua-ning Wang,Qingrong Tan(摘要未译)YRD
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rTMS Ameliorates PTSD Symptoms in Rats by Enhancing Glutamate Transmission and Synaptic Plasticity in the ACC via the PTEN/Akt Signalling Pathway Gaohua Liu1 & Dayun Feng2 & Jian Wang3 & Haifeng Zhang2 & Zhengwu Peng1 & Min Cai1 & Jing Yang2 & Ruiguo Zhang1 & Huaning Wang1 & Shengxi Wu2 & Qingrong Tan1 Received: 2 February 2017 /Accepted: 4 May 2017 # Springer Science+Business Media New York 2017 Abstract Repetitive transcranial magnetic stimulation (rTMS) is a novel physiological therapy that has been adopted to clinically treat psychiatric disorders. Our previous study indicated the potential therapeutic effect of rTMS on posttrau- matic stress disorder (PTSD). However, the exact molecular mechanism is elusive. Currently, using the single prolonged stress (SPS) rat model for PTSD, we investigated the gluta- matergic transmission and neural plasticity changes in the an- terior cingulate cortex (ACC) after SPS induction and ex- plored the protective effects and mechanism of rTMS treat- ment. We found that high-frequency rTMS (HrTMS, 15 Hz) treatment significantly relieved the impaired glutamatergic re- ceptors in the ACC after SPS treatment by significantly in- creasing NMDAR and AMPAR expression. Simultaneously, HrTMS blocked inhibited neuronal phosphatase and tensin homologue on chromosome 10 (PTEN)/Akt signalling in the ACC after SPS treatment by decreasing PTEN expression and increasing Akt phosphorylation, which is critically involved in the regulation of memory and synaptic plasticity. The PTEN inhibitors bpV and small interfering RNA and the Akt inhibitor wortmannin were stereotaxically administered to the ACC after SPS treatment to advance the mechanistic study. Analysis by Western blot, double immunofluorescence, Golgi staining and behavioural tests demonstrated that the effects of rTMS on PTEN/Akt activation, glutamatergic receptor ex- pression, neuronal synaptic plasticity and PTSD-related be- haviours induced by SPS treatment were enhanced by PTEN inhibition and blocked by Akt inhibition in the ACC. Our study provides convincing evidence for the effectiveness of rTMS treatment on PTSD and suggests that its potential mech- anism involves remodelling neuronal synaptic plasticity via the PTEN/Akt signalling pathway. Keywords PTSD . rTMS . ACC . PTEN . Akt . Glutamate receptor Introduction Posttraumatic stress disorder (PTSD) was first recognized by the American Psychiatry Association in the 1980s, and it is characterized by flashbacks, re-experience, hyperarousal, avoidant behaviour and numbed emotions [1]. This disorder has a high prevalence rate among the general population (ap- proximately 1%), and PTSD considerably affects an individ- ual’s health, social and occupational function, family and overall quality of life [2]. To date, first-line treatment for PTSD includes selective serotonin reuptake inhibitors (SSRIs), psychotherapy, such as prolonged exposure therapy [3] and psychodynamic psy- chotherapy [4]. These therapies have a certain effectiveness Liu GH, Feng DY and Wang J contributed equally to this work. Electronic supplementary material The online version of this article (doi:10.1007/s12035-017-0602-7) contains supplementary material, which is available to authorized users. * Shengxi Wu shengxi@fmmu.edu.cn * Qingrong Tan tanqingr@fmmu.edu.cn 1 Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, People’s Republic of China 2 Department of Neurobiology and Institute of Neuroscience, Fourth Military Medical University, Xi’an 710032, People’s Republic of China 3 Department of Cardiac Surgery, General Hospital of Chengdu Military Region, Chengdu 610083, People’s Republic of China Mol Neurobiol DOI 10.1007/s12035-017-0602-7

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